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TOXICITY PLATFORM (NicTox™)

Toxicity testing for next generation nanomaterials

The platform aims to test the toxicity of nanomaterials produced in-house and outside, for first line exposure to scientists and workers; along with specific toxicity testing for engineered nanomaterials with specific application.  Toxicity has become a major concern in nanotechnology advancement and along with the Nanoscience and Nanotechnology Code of Conduct, the testing of nanoparticle and nanomaterial toxicity has reached national importance.


The NIC Toxicity Platform (NicTox™) aims to investigate cellular toxicity as a first screening towards understanding, not only the mode of toxicity, but the reason for the toxicity.  Testing is available as end point assays, but more importantly, in real time with the use of the xCELLigence. Various normal and cancerous cell lines are available for toxicity elucidation.

CAPABILITIES

  • Normal, as well as cancerous cell line testing (specific cell lines can be sourced on request)
  • Consecutive real time toxicity testing of 24 compounds, in triplicate, on any of the cell lines
  • Toxicity typing with reference materials
  • Dose dependent studies
  • Induced toxicity mitigation studies
  • Comparative end-point assay assessment
  • Apoptosis/Neucrosis determination

SERVICES OFFERED

END POINT ASSAYS

Various end point assays are available for testing.  MTS assays are preferred and tested in conjunction and as comparison to real time toxicity testing.

REAL TIME TOXICITY TESTING

Real time toxicity testing with  the xCELLigence  system.   The  xCELLigence system is a real time cell based analysis system  that uses  impedance as a measure  of cell  viability.  The  system  uses gold  electrode coated  96-well plates to measure current across the base of a single well. 

A  living  adherent  cell,  with  active  metabolic  function,  causes resistance when adhered to the  well surface, producing a visible graph.  Any change in this  adherence;  a  loss  in  adherence  strength,  curling  of cell edges and a total loss  of adherence, due  to cell death, can be  visualised on the system, in real time.

The growth pattern for each cell line is unique, as is the cellular response to the  introduced  toxin.  Due to  the  nature  and  sensitivity  of  the  real  time xCELLigence system, specific types of toxicity can be identified. 

CURRENT CELL LINES

NORMAL CELL LINES

ARPE-19        Pigmented retinal epithelium
BHK-21          Kidney fibroblasts, HAMSTER
CCD 841        CoN Colon epithelials
Detroit 551    Skin fibroblasts
HEL299          Lung fibroblasts
MCF12A        Breast epithelials 
MRHF             Dermal fibroblasts
Mv1Lu            Lung epithelials, MINX
RD                   Muscle cells

CANCEROUS CELL LINES

A549           Lung carcinoma
A-673         Muscle, Ewing's Sarcoma 
Caco-2       Colon, Colorectal adenocarcinoma 
Caki-1        Kidney: skin, Clear cell carcinoma 
H69AR       Multidrug resistant lung carcinoma 
HCT116     Colorectal carcinoma 
Hek-293    Kidney, embryonic, Adenovirus
HeLa          Cervical adenocarcinoma
Hep-2         Larynx, Eperimoid carcinoma
Hep-G2      Liver, Hepatocellular carcinoma 
HT-29        Colorectal adenocarcinoma 
HT-3          Cervical retinoblastoma 
MCF10A    Breast; Fibrocystic disease 

CANCEROUS CELL LINES

MCF7            Breast; Adenocarcinoma
NCI-H226     Lung; Squamous cell carcinoma 
NCI-H358     Lung; Bronchioalveolar carcinoma 
PC-3              Prostate; Adenocarcinoma
RPMI-7951  Skin; Malignant melanoma (lymph)
SK.MEL2      Skin; Malignant melanoma (thigh)
Sk-OV-3       Ovary; Resistant adenocarcinoma
T47D             Breast; Ductal carcinoma 
TE 354.T      Skin, Basal cell carcinoma 
TK-10           Kidney, Renal adenocarcinoma
TOV-21G     Ovary; malignant adenocarcinoma 
U-251           Brain, Glioblastoma, Astrocycoma

CONTACT US FOR MORE INFORMATION